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1 Tirzepa�de deriva�ves Tirzepa�de (CAS-No. 2023788-19-2) is a glucose-dependent insulinotropic polypep�de and glucagon-like pep�de-1 receptor agonist. According to the custom in the field, the sequence of amino acids is writen from N-terminus (le�) to C-terminus (right), any subs�tuents to an amino acid side chain are indicated in brackets () to the right of the amino acid concerned, and the amino acids are in L-configura�on unless otherwise specified. The “H-“ at the N-terminus indicates a free amino group, the “- NH2” at the C-terminus indicates a carboxamide group and the “-OH” at the C-terminus indicates a carboxylic acid group. Posi�ons along the pep�de chain may be given by numbers star�ng with 1 at the N-terminus and may be indicated in superscript to the right of the amino acid concerned. Posi�ons within the side chain subs�tuent of an amino acid start with 1 for the moiety atached to the side chain of the amino acid and may be indicated with a dot in superscript to the right of the amino acid concerned. For example, in case of a Lys at posi�on 20 as the amino acid, the moiety atached to the epsilon-nitrogen atom of Lys is numbered with “moiety20.1”. For prac�cal reasons, a “moiety” covers an amino acid as well as a non- amino acid, which is bound via an amide bond, e.g., a faty acid. For the sake of brevity, some or almost all or all posi�on indicators may be le� out, without changing the informa�on. Formula I is Tirzepa�de’s molecular structure expressed in the three-leter-code with an individual numbering of all amino acids: H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17- Ala18-Gln19-Lys20(AEEAc20.1-AEEAc20.2-gamma-Glu20.3-19-carboxy-nonadecanoyl20.4)-Ala21- Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37- Pro38-Ser39-NH₂ (I) Formula II is Tirzepa�de’s molecular structure expressed in the three-leter-code without an individual numbering of all amino acids: 2 H-Tyr-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Ile-Aib-Leu-Asp-Lys-Ile-Ala-Gln-Lys(AEEAc- AEEAc-gamma-Glu-19-carboxy-nonadecanoyl)-Ala-Phe-Val-Gln-Trp-Leu-Ile-Ala-Gly-Gly-Pro- Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH₂ (II) In both formulae I and II, AEEAc represents the amino acid 8-amino-3,6-dioxa-octanoic acid. Formula I and formula II above describe the same compound, i.e., Tirzepa�de. In the following, Tirzepa�de is compound No. 100. It will be understood that a pep�de such as Tirzepa�de or a Tirzepa�de deriva�ve may be present as a salt with various counter-ions both at side chain func�onal groups and terminal func�onal groups. A numbering of an amino acid is used in the following in case a reference is made to a posi�on of the amino acid. Tirzepa�de deriva�ves are of interest as pharmaceu�cal compounds, as some may be expected to have similar physiological effects as Tirzepa�de. As such, they might be suitable for treatment of metabolic diseases, for example to improve glycemic control in adults with type 2 diabetes or for weight management. A synthesized Tirzepa�de deriva�ve allows an evalua�on of its physiological effects including its applica�on in respec�ve in-vitro test system. Moreover, small amounts of Tirzepa�de deriva�ves may be comprised in a pharmaceu�cal composi�on containing Tirzepa�de as a desired drug substance. For example, the material obtained from a manufacturing process of Tirzepa�de, which is used as the drug substance in a prepara�on of a pharmaceu�cal composi�on containing Tirzepa�de as a desired drug substance, contains already small amounts of Tirzepa�de deriva�ves. A synthesized Tirzepa�de deriva�ve supports an analy�cal determina�on of the Tirzepa�de deriva�ve assumed to be in a pharmaceu�cal composi�on containing Tirzepa�de as a desired drug substance or in the material obtained from a manufacturing process of Tirzepa�de. These are reasons, why synthesis of Tirzepa�de deriva�ves is of interest. Overview of Tirzepa�de deriva�ves Some of the Tirzepa�de deriva�ves can be assigned to certain categories due to their way of differing from Tirzepa�de. A way of differing can be a missing amino acid, i.e., a dele�on (des). Similarly, a way of differing can be an addi�onal amino acid, which is a doubling of what should be solely one amino acid, i.e., an addi�on (endo). Another way of differing can be an exchange 3 of one amino acid with a different amino acid, i.e., a subs�tu�on. Another way of differing can be a change of an amide group into a carboxylic acid group, i.e., a deamida�on. Another way of differing can be a missing of one or more amino acids at the N-terminal end of Tirzepa�de, i.e., a trunca�on. There are cases, where one and the same difference of a Tirzepa�de deriva�ve from Tirzepa�de can be assigned to two different categories. For example, a deamida�on of Gln to Glu is also a subs�tu�on of Gln by Glu. For example, a trunca�on is a specific dele�on, i.e., a dele�on at the N-terminal end. Compounds, which are Tirzepa�de deriva�ves, are listed in th...